Fumigation methods and phytosanitary plant protection for greenhouse soil
18 min read
Soil disinfection: protection against pathogens and prevention of toxicoses
Effective phytosanitary preparation of greenhouse soils minimizes the risk of crop infection throughout the entire season. The use of methyl bromide helps to reliably clean the substrate of major pests and disease agents, including root-knot nematodes. However, when working with fumigants, it is important to strictly observe dosage rates and consider the consequences of sterilization for soil microflora.
- Dose for microorganism suppression — 50 µg/ml
- Application rate against root-knot nematode — 80 g/m²
- Interval between cucumber crop rotations — at least 3–4 weeks
| Microorganism type | Result of exposure (dose 50 µg/ml) |
|---|---|
| suppression | |
| suppression | |
| suppression | |
| suppression | |
| suppression | |
| suppression | |
| suppression | |
| in tomato stems | suppression |
For reliable plant protection against root rots in fumigated soils, an agronomist needs to perform two consecutive operations. These allow for closing the main pathways of infection spread and restoring the balance of the microflora.
- Steaming or fumigation of the transplant medium as a potential source of infection.
- Restoration of the biological diversity of soil microflora using manure or humus.
The application of methyl bromide at a rate of 80 g/m² effectively suppresses all soil-borne pathogens. However, after fumigation, saprotrophic bacteria remain in the soil and begin to multiply rapidly in the clean substrate. Their metabolic products cause soil toxicoses, which lead to the death of some of the root hairs in transplants and cause the plants themselves to lose resistance.
To reduce the negative aftereffects of soil disinfection and avoid toxicoses, well-rotted manure or humus should be applied strictly after steaming or fumigation, and not before.
Protective measures are always planned taking the previous crop into account. For example, a winter-spring cucumber crop can be preceded by tomato, extended or autumn cucumber, leafy greens, eggplant, or pepper. The complex of harmful organisms accumulated on them persists in the greenhouse and affects new plantings. Autumn cucumber following a winter-spring crop suffers severely from mosaic, ascochyta blight, phoma root rot, root rots, and powdery mildew, which require a technological break for prevention.
Phytosanitary role of drip irrigation in a greenhouse
The method of water supply directly determines the humidity level of the air and the substrate in the greenhouse. Traditional irrigation systems often trigger infection outbreaks due to sharp fluctuations in the microclimate. Modern drip irrigation helps to automate the processes of plant nutrition and simultaneously improve the overall phytosanitary conditions.
In modern greenhouse farms, water or nutrient solutions are supplied in several ways:
- surface irrigation;
- hose irrigation;
- sprinkler irrigation using nozzles;
- drip irrigation.
With traditional overhead or hose irrigation, optimal humidity is created only periodically. Immediately after water application, the substrate becomes waterlogged, air does not reach the roots, which weakens their absorptive function and opens the way for pathogens. Intensive evaporation of moisture from the leaves sharply increases air humidity, provoking morning guttation and creating an ideal environment for spore germination.
Drip irrigation using "Supertif" or "Hydrogol" emitters delivers the solution directly to the substrate, keeping the above-ground part of the plants and the air dry. This prevents the development of fungal diseases such as gray mold on tomato, pepper, eggplant, and ascochyta blight on cucumber. If the technology requires increasing air humidity, an overhead misting system with "Rondo" micro-sprinklers is used, which creates a semi-fog in 5–7 seconds of operation.
The use of an automatic analytical system for recording drainage and irrigation water from the AL.K company allows for precise control of nutrient uptake. Computer analysis of this data helps to prevent physiological disorders, such as blossom-end rot of tomato and pepper fruits.
Drip irrigation is most often used when growing plants in small-volume substrates: in containers, in pots, in heat-resistant Mapal trays on racks, on peat slabs, on Grodan, and on other mineral and organic substrates. The transition to small-volume substrates is driven not only by economic calculations but also supported by phytosanitary considerations. For example, the transition to this technology allows solving the problem of controlling a number of dangerous pathogens, such as root-knot nematodes, and certain root rot pathogens.
Drip irrigation is also used for the prophylactic application of systemic preparations, such as Previcur, Aktara, or Confidor, which allows protecting plants from both pests and diseases. The application of these preparations in the greenhouse can be carried out by one person, saving time and labor. Moreover, these preparations can be applied periodically, protecting plants throughout the growing season via the drip irrigation system with minimal labor requirements.
Mineral nutrition of vegetable crops
Soil preparation, maintaining an optimal microclimate, proper care, and plant nutrition contribute to increasing their resistance to pests and diseases. When developing rational crop nutrition plans, one should proceed from the main strategic line: plants should never starve; moreover, one should never apply less than the amount of fertilizer that a crop can assimilate. Increased quantities of Ca, B, Cu, Zn, Mn, and Mo by 10-15% are necessary, as this contributes to increasing plant disease resistance to root rots and certain other diseases. Consequently, the key to success is the use of high-quality fertilizers. Modern greenhouse vegetable production places increasingly higher demands on the economic side of farm performance. Stability and the production of high-quality, competitive produce come to the fore. A great deal depends on mineral nutrition, especially when enterprises switch to modern low-volume growing systems. In low-volume substrates, practically all nutrients are applied with the irrigation solution, while basal dressing is used only in soil-based greenhouses in combination with the application of dolomite flour as a source of cheap Ca and Mg nutrients.
Under current conditions, the prices of mineral fertilizers offered to Russian greenhouse enterprises by various manufacturers are at approximately the same level. Therefore, the quality of fertilizers becomes of particular importance: their composition balance, ease of use, reliability, and stability. They must contain all the nutrients necessary for the plant and strictly correspond to the stated composition. As an example, let us consider the assortment of fertilizers offered on the Russian market by ZAO Kemira Agro. It includes all necessary fertilizers, both for drip irrigation and for soil-based greenhouses, meeting high standards; therefore, most greenhouse complexes, by using them, obtain a high-quality harvest and, consequently, a noticeable economic effect.
Nutrition systems for vegetable and flower crops using the low-volume growing method are based on the application of one complete water-soluble fertilizer and several simple fertilizers as a base:
Kemira Kombi is a complete fertilizer for peat substrates with a high content of microelements in the form of chelated compounds. This is an acidic fertilizer, the application of which into peat as a basal dressing together with dolomite flour creates optimal soil acidity at the pH level (5.8-6.2) due to the fact that in an acidic environment, such nutrients as Ca and Mg contained in dolomite flour become more accessible to plants. As a result, the consumption of expensive soluble calcium and magnesium fertilizers and acids is noticeably reduced.
Kemira Hydro is a complete, soluble, chlorine-free fertilizer with a high content of microelements in the form of chelated compounds, designed for use on any substrate. On its basis, one can obtain a nutrient solution with various element ratios.
Calcium nitrate is a simple fertilizer containing water-soluble calcium, which makes it indispensable when growing plants using the low-volume hydroponics method.
Potassium sulfate is an impurity-free, chlorine-free, almost completely water-soluble (at least 99.8%) simple fertilizer, which makes it suitable for drip irrigation.
Potassium nitrate is ideal for greenhouse crops, both due to its high quality and thanks to its convenient packaging and resistance to caking during storage.
TABLE 13. FORMULATIONS OF NUTRIENT SOLUTIONS for CUCUMBER AND TOMATO CROPS WHEN GROWN ON PEAT HCO3- - 2.3mmol, Ec -0.2mS/cm Resulting working nutrient solution Ec 2.8, pH - 5.6-5.8 in mg/l, MK 1:1.4 N Ca Mg K [in mg/l] [284] [43] [391] [176] [64] [36] [18] [0.9] [0.14] [0.4] [0.2] [0.02] [0.01] Solution for cucumber crop on peat (For 1000l of 1:100 stock solution). For raw water containing Ca - 0.8mmol, Mg - 0.6mmol, HCO3- - 2.3mmol, Ec 0.2mS/cm Kemira Kombi Nitric acid 68% Conductivity Ec mS/cm Resulting working nutrient solution Ec - 2, pH - 5.6-5.8 in mg/l, MK 1:1.1. [in mg/l] 0.013 | 0.007
Magnesium nitrate, being a liquid fertilizer, contains water-soluble magnesium, which ensures ease of use. As an example, the formulation for preparing a nutrient solution for top dressing cucumbers and tomatoes in low-volume culture should be cited.
Fertilizers of the "Kemira Agro" line contain chromium, iodine, cobalt, and selenium. These microelements are not vital for the plants themselves, but they are actively assimilated by them. As a result, the grown vegetable produce is enriched with valuable substances, acquiring dietary properties.
For base application and top dressing of vegetables and flowers in open ground and greenhouses, granulated complexes "Kemira Universal" and "Kemira Universal-2" are effective. Nutrients reach the roots gradually throughout the entire growing season. This prevents nutrients from leaching out of the root zone, increases their utilization rate, saves resources, and reduces environmental impact.
"Kemira Universal" and "Kemira Universal-2" fertilizers are poorly soluble. They must not be applied through the irrigation system; the granules should be incorporated directly into the soil.
"Kemira Super" complex fertilizer has been specifically developed for amending peat substrates. It contains microelements both in a rapidly available chelated form and as slowly soluble carbonates. To reduce plant stress during unfavorable weather, mineral top dressing is combined with growth regulators and hormones, which adjust hormonal balance and stimulate yield.
| Fertilizer | N | P₂O₅ | K₂O | Mg | Ca | Fe* | Mn* | Cu* | Zn* | Mo | B | EC (0.1%) | Solubility, % |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Hydro Kemira | 14 | 11 | 26 | 1.4 | 0.1 | 0.1 | 0.01 | 0.01 | 0.02 | 0.002 | 0.001 | 1.25 | 100 |
| Combi Kemira | 11 | 24 | 24 | 0.5 | 0.4 | 1.0 | 0.2 | 0.08 | 0.10 | — | — | — | — |
* Iron, zinc, copper, manganese, and cobalt are in the form of EDTA chelates.
Phytosanitary seed and planting material preparation
Seed lots completely free from latent infection are rare. Pathogens of fusarium wilt and bacterial diseases are easily transmitted via seeds, whereas grey mold does not spread this way. In tomatoes, the most dangerous pathogen is the causative agent of pith necrosis of the stem (Pseudomonas corrugata).
- Baktofit dosage for tomato seeds — 1.5–5 kg/t
- Phytolavin-300 working solution — 0.2%
- Soaking time in Phytolavin — 2 hours
- Phytolavin-300 application rate — 10 g/kg of seeds
- Cucumber seed infestation — 5–35%
Seed treatment with Baktofit reduces the manifestation of primary pith necrosis foci but does not stop the subsequent secondary spread of the disease.
For the control of tomato vascular bacteriosis, the antibiotic griseofulvin is used, though it does not guarantee complete elimination of the infection. Cucumber seeds are often infested with pathogens of fusarium, pythium, ascochyta, and downy mildew. Conventional surface disinfection with potassium permanganate does not remove internal seed infestation.
Arcerid is effective against fungi of the genus Pythium on cucumbers; other fungicides do not cope with this pathogen. For mixed infection control, tank mixtures are used: for example, Apron Gold reliably suppresses seed-borne downy mildew, and TMTD eliminates bacterial infection.
Some viruses lose viability inside seeds naturally during storage. This allows for the use of simple agrotechnical methods to improve planting material without chemical stress. For instance, the infestation of seeds with cucumber green mottle mosaic virus critically drops over time.
- Use cucumber seeds that have been stored for two years for sowing — the green mottle mosaic virus is not transmitted through them.
- In the absence of two-year-old seeds, disinfect the planting material using the heat treatment method.
To perform heat treatment, it is necessary to have two thermostats, which are set to the different indicated temperatures before loading the seeds. The thermostat chamber and shelves must have holes located 1-2 cm apart for uniform heat distribution. The shelves are covered with gauze, on which seeds are spread in a layer no more than 1 cm thick and dried well at room temperature for 1-2 days. Before loading the entire batch of seeds into the thermostat for heating, it is necessary to take 50-100 seeds from it, process them in the specified manner, and then germinate them. If germination decreases significantly after heating, it means the seeds were moist; therefore, the main seed lot must be additionally dried at 30-40° for a day before being processed in the thermostat. The seeds are first heated for three days at an air temperature of 50-52°, and then immediately moved to a chamber with a temperature of 78-80° for one day, which guarantees their freedom from viruses. Seeds treated according to this method do not show reduced germination, although they sprout 1-2 days later than the control group. This is due to the loss of a portion of moisture during heating, which is needed for germination. Therefore, treated seeds must be thoroughly moistened before germination by soaking them in water or a microelement solution for 12-24 hours, and then germinating at a temperature of 25-28°. Heating seeds also reduces their infestation by 3-5% with such disease pathogens as root rots and fusarium, but has practically no effect against ascochyta. 3.2.6. Agrotechnical method of controlling root-knot nematodes Over many years, various agrotechnical methods have been developed and tested in our country and abroad to effectively control the population of root-knot nematodes in protected ground (Kiryanova, Kral, 1969; 1971; Sasser, Carter, 1985). The application of organic and mineral fertilizers, the use of resistant and trap crops, and the application of predatory fungi and other nematophagous microorganisms were studied. In almost all cases, positive results were obtained, allowing for the suppression of meloidogyne development, but none of these methods, taken individually, contributed to the process of complete soil disinfection. Only a strict sequence of anti-nematode measures and time intervals between crop rotations can lead to complete soil disinfection in greenhouses.
Application of organic fertilizers and maintaining optimal soil moisture facilitate the rapid decomposition of large galls remaining after root removal due to an increase in the overall biological activity of the soil. In particular, the development of predatory fungi and other microorganisms is stimulated. The application of organic fertilizers has a positive effect on the population of predatory nematodes and soil arthropods. To significantly improve the structure and biological activity of the soil, the application rate of organic fertilizers must be at least 120 t/ha.
Provocative irrigation of the soil with a water extract from a host plant facilitates the emergence of invasive larvae from a state of anabiosis and increases their locomotor activity aimed at searching for the roots of the host plant. As a result, some larvae, having exhausted their fat reserves without finding a host plant, perish. To obtain a 0.1% solution of host plant sap, non-standard fruits are used, as well as the above-ground parts of cucumber or tomato plants (can be used in a mixture).
Sowing a trap crop (which is also a valuable green manure crop) is a highly effective technique in the scheme of anti-nematode measures. The use of this method is based on the fact that the surviving invasive larvae penetrate the root system of the trap crop and, without completing their development, perish after the host plant is destroyed (ploughed under).
An essential condition for any system of anti-nematode measures is weed control, since the hosts of only three species of root-knot nematodes parasitizing vegetable crops in greenhouses in Russia include more than 400 species of herbaceous plants (Kiryanova, Krall, 1969, 1971).
| n | aa | Anti-nematode measures and optimal soil moisture |
| 1 | 1.8-15.8 o | Soil tillage and weed control_ optimal soil moisture |
| 8 | 1 | 22.859 [| Soil tillage and weed control_ |
| 9 | 169449 | Sowing a trap crop. Maintaining optimal soil moisture |
When planning anti-nematode measures, it should be kept in mind that the lifespan of the invasive stage of root-knot nematodes in the absence of a host plant, even under favorable external conditions for the parasite (low temperature and low soil moisture), does not exceed eight months. From a production point of view, the most suitable time for carrying out the entire complex of anti-nematode measures in the Central and Central Black Earth regions of Russia is the second half of summer after the completion of the first crop rotation.
Below is the most effective scheme of agrotechnical anti-nematode measures in greenhouses, which has been tested in the conditions of the Central region of the Russian Federation.
The use of trap (green manure) crops is a reliable way to clean greenhouse soil of root-knot nematodes. The working principle is simple: the pest larvae penetrate the roots of the young green manure, after which the plants are incorporated into the soil or removed before the nematode has time to complete its development cycle and produce offspring. Large-seeded legumes are best suited for this purpose: peas, soy, or beans. Small-seeded legumes, such as clover or annual lupine, show significantly lower efficiency.
- Sowing rate of green manure crops — 150–250 seed/m²
- Distance between trap plants — up to 10 cm
- Migration distance of larvae in the soil — up to 5–7 cm
- Temperature for a rapid development cycle — 26–28 °C
- Average daily temperature in September — 18–22 °C
- Growing season in the middle latitudes — 25–28 days
The effectiveness of the technique directly depends on the timing: the longer the interval between the removal of the main crop residues and the sowing of the green manure, the better the result. In warm conditions at an average daily temperature of 26–28 °C, the development cycle of the root-knot nematode takes 18 days. It is precisely this period that is allotted for the growth of the green manure under standard conditions.
In the conditions of the middle latitudes of Russia, it is optimal to sow the trap crop at the beginning of September. Since the average daily temperature in the greenhouse drops to 18–22 °C during this period, larval activity decreases and seeds germinate more slowly. Under these conditions, the pest's development cycle is extended, so the growing season of the trap crop must be extended by 7–10 days. The total cultivation period for the green manure will be 25–28 days — during this time, new egg sacs will not have time to form.
In the last 5–7 days of the green manure's growth, be sure to monitor the roots of the plants. Do not allow the formation of nematode egg sacs, otherwise, instead of cleaning the soil, you will get a pest outbreak.
After finishing the cultivation and incorporation of the green manure, move on to preparing the greenhouse for the first rotation of the new season. It is important to strictly follow the sequence of technological operations. Violating the steps will reduce the overall phytosanitary effect.
- Thermal disinfection (steaming) of the soil.
- Application of mineral and organic fertilizers.
In addition to destroying nematodes, a clean six-month fallow helps to sanitize the soil. It effectively combats soil fatigue, reduces salinity, and suppresses soil pests and pathogens of Fusarium root rot. Incorporating green manure biomass improves the physical structure of the soil and stimulates beneficial saprotrophic microflora.
Choosing crops for subsequent rotation and biological protection
The success of all treatments performed depends on which plant will occupy the greenhouse in the next growing season. The correct choice of the subsequent crop helps to consolidate the result or nullifies all efforts. The crop rotation scheme determines the phytosanitary condition of the soil for the entire year.
Planting cultivars and hybrids of tomato resistant to root-knot nematode significantly enhances the effect of disinfection. Planting cucumber in the same rotation, on the contrary, sharply reduces the effectiveness of all the work done.
If, due to organizational or technical difficulties, the farm cannot complete the entire technological chain, the system may be simplified. It is permissible to exclude the planting of trap crops or the application of organic fertilizer from the scheme. The overall anti-nematode effect will decrease, but the final level of soil disinfection will still remain sufficiently high.
When integrating biological plant protection products, use only officially approved preparations. All treatments must strictly comply with state standards and methods. In biological protection, two practical factors play a key role: the quality assurance of the biomaterial from the supplier and strict adherence to application regulations.
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